L. SCHERGER, S. BROCKLEHURST, N. FUSELIER, M.N. RYLANDER
The purpose of this research was to design an in-vitro biomimetic skin platform that accurately mimics native skin tissue that can be used to study the cellular response to burns. Biomimetic platforms are important for burn treatment development because they offer an inexpensive, ethical method for experimentation, which minimizes reliance on animal models. During this Connecting Experience, the study optimized 3D bioprinting skin by experimenting with the makeup of the bioink (the base material for printing), the cellular complexity of the skin model, and the design of the platform. The ECM bioink made of high concentration type 1 collagen with Primary Human Dermal Fibroblasts was successfully printed suspended within a gelatin support bath. The printed skin model retained shape and sterility for 3 days in incubation. The future direction of this research will continue to optimize printability for 3D models to create vessels with specific wall thicknesses that can withstand microfluidic pumping of cell media to more accurately represent native skin tissue vascularization.